Invitrogen 18080-044 反转录酶III SuperScript III Reverse Transcriptase

Invitrogen 18080-044 反转录酶III  SuperScript? III Reverse Transcriptase 
货号： 18080-044
Invitrogen?  相关应用： 反转录和 cDNA 合成
货号	规格	价格
18080-044	10,000 units	4,245.00
18080-085
4 x 10,000 units	15,350.00
18080-093
2,000 units	1,220.00

描述
SuperScript? III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript? II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A)+ RNA sample. Like SuperScript? II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. SuperScript? III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability.

? Thermostability – Half life of 220 minutes at 50°C
? Yield – Reduced RNase H activity for more full-length cDNA
? Specificity – Full activity at 50°C for increased specificity with Gene Specific Primers
? Applications – Array labeling, cDNA libraries, RT-PCR, primer extension, and 3′ and 5′ RACE

Source: Purified from E. coli expressing the pol gene of M-MLV (1, 2), mutagenized to increase thermal stability and half life and reduce RNase H activity.

Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays (Figure 2) and yield and length of cDNA product.

Unit Definition: One unit of SuperScript? III RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)?oligo(dT)12-18 as a template?primer (3).

Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, and enzyme in 50 μl for 10 min. at 37°C.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
规格
Optimal Reaction Temperature:	50° C
Final Product Size(s):	12.3 kb or less
Reverse Transcriptase:	SuperScript? III
Ribonuclease H Activity:	Reduced
Form:	Frozen
Format:	Tube(s)
Quantity:	10,000 units
Template:	ssRNA
Sensitivity:	Medium
Product Size:	10,000 units
Concentration:	200 U?μl
Enzyme Function:	RNA dependent DNA Polymerase
Shipping Condition:	Dry Ice
Number of Reactions:	50 Reactions

内容及储存
SuperScript? III Reverse Transcriptase is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial of 100 mM DTT.
Store at -20°C. Guaranteed stable for 6 months when properly stored.

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